Figure 2.

Western blotting of astrocyte extracts with monoclonal antiperforin antibody. Detergent extracts of a rat NK (RNK) cell line (positive control), rat neonatal astrocytes, and human fetal astrocytes (passage 7, 2 × 10⁷ cells/ ml) were run on SDS-PAGE (10% acrylamide gel), Western blotted, and probed with the monoclonal antiperforin antibody (0.1–0.2 μg/ml). Bound antibody was detected using goat anti–mouse immunoglobulins (1 out of 1,000) and developed using the enhanced chemiluminescence detection system. A major band at 65 kD in the rat NK cell lysates was the predicted size for perforin. A single major band of 65 kD was also detected in lysates of human fetal astrocytes (arrowed) but not in neonatal rat astrocytes (28).