Table 1.
Negative Ion ESI-MS Data and Proposed Compositions for O-deacylated LPS from TEPC-15–nonreactive and –reactive Colonies of Type b H. influenzae (RM 7004)
| LPS glycoform | Observed [M-3H]3− ions* | Molecular mass | Proposed composition | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TEPC-15 non-reactive | TEPC-15 reactive | |||||||||
| Observed | Calculated‡ | |||||||||
| Hex3 | 812.0 | 812.1 | 2439.0 | 2439.2 | Hex3 × Hep3 × Pea1 × KDO1 × P1 × LipidA-OH | |||||
| 866.7 | 2603.1 | 2604.3 | ChoP × Hex3 × Hep3 × PEA1 × KDO1 × P1 × | |||||||
| LipidA-OH | ||||||||||
| Hex4 | 865.9 | 865.7 | 2601.1 | 2601.3 | Hex3 × Hep3 × Pea1 × KDO1 × P1 × LipidA-OH | |||||
| Hex4 × Hep3 × PEA2 × | ||||||||||
| 907.0 | 2724.0 | 2724.5 | KDO1 × P1 × LipidA-OH | |||||||
| 920.7 | 2765.1 | 2766.3 | ChoP × Hex4 × Hep3 × PEA1 × KDO1 × P1 × | |||||||
| LipidA-OH | ||||||||||
| 962.0 | 2889.0 | 2889.4 | ChoP × Hex4 × Hep3 × PEA1 × KDO1 × P1 × | |||||||
| LipidA-OH | ||||||||||
| Hex5 | 919.9 | 919.8 | 2762.4 | 2763.5 | Hex5 × Hep3 × PEA1 × KDO1 × P1 × LipidA-OH | |||||
| Hex5 × Hep3 × PEA1 × | ||||||||||
| 961.0 | 2886.0 | 2886.5 | KDO1 × P1 × LipidA-OH | |||||||
| 975.0 | 2928.0 | 2928.6 | ChoP × Hex5 × Hep3 × PEA1 × KDO1 × P1 × | |||||||
| LipidA-OH | ||||||||||
| 1016.0 | 3051.0 | 3051.5 | ChoP × Hex5 × Hep3 × PEA2 × KDO1 × P1 × | |||||||
| LipidA-OH | ||||||||||
| Hex6 | 974.1 | 974.0 | 2025.0 | 2925.6 | Hex6 × Hep3 PEA1 × KDO1 × P1 × LipidA-OH | |||||
| Hex6 × Hep3 × PEA2 | ||||||||||
| 1015.0 | 3048.0 | 3048.6 | KDO1 × P1 × LipidA-OH | |||||||
| 1029.0 | 3090.0 | 3090.7 | ChoP × Hex6 × Hep3 × PEA1 × KDO1 × P1 × LipidA-OH | |||||||
| Hex7 | 1027.8 | − | 3086.4 | 3087.8 | Hex7 × Hep3 × PEA1 KDO1 × P1 × LipidA-OH | |||||
| Hex8 | 1081.6 | − | 3247.8 | 3249.9 | Hex8 × Hep3 × PEA1 × KDO1 × P1 × LipidA-OH | |||||
| Hex9 | 1136.1 | − | 3411.3 | 3412.0 | Hex9 × Hep3 × PEA1 × KDO1 × P1 × LipidA-OH | |||||
*
The spectra were dominated by molecular peaks corresponding to triply deprotonated ions.
‡
Average mass units used for calculation of molecular mass values based on proposed compositions as follows: Hex, 162.15; Hep, 192.17; KDO, 220.18; P, 79.98; PEA (phosphoethanolamine), 123.05; ChoP, 165.05.