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. 1998 Mar 2;187(5):795–799. doi: 10.1084/jem.187.5.795

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(A) Induction of RAG gene expression in activated mouse mature B cells in vitro. Mouse spleen B cells were cultured with LPS or LPS plus IL-4 for 2 d, and assessed for RAG-2 expression by RT-PCR. (B) Enhanced expression of λ chains in mouse spleen B cells stimulated with LPS/IL-4. After stimulation with LPS or LPS/IL-4 for 4 d, B220⁺ population of the cultured B cells was analyzed for the expression of λ, μ, and ^γ1 chains by flow cytometry.