Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Mar 2;187(5):787–794. doi: 10.1084/jem.187.5.787

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Detection of ST2L message in Th2s. RNA from a panel of four each of different Th1 (Dorris, X4, X9, and X17) and Th2 (D10, D2.2, D2.3, and X12) clones (resting cells, two wk after antigen stimulation) were extracted and compared by DD-PCR. (a) Two representative patterns comparing the PCR products from Dorris (Th1) and D10 (Th2) cells, using two different primer pairs (lanes 1 and 2, H-T11A/H-AP34; lanes 3 and 4, H-T11G/H-AP34). Arrowhead, the ST2L band. (b) Nucleotide sequence of one cDNA present in Th2s, but not in Th1s. This has complete homology with the 3′ terminus of ST2L cDNA. (Data are available from EMBL/GenBank/DDBJ under accession number D13695.) The primer (H-T11A/H-AP34) binding sites are underlined. (c) Northern blot analysis detected ST2L in D10, but not in Dorris cells. Similar results were obtained for the other three Th1 and Th2 clones (not shown). (d) Reverse transcriptase PCR Southern demonstrates the presence of ST2L message in Th2, but not in Th1, clones. c and d are representative of three experiments.