Figure 5.

Anti-ST2L antibody exacerbated collagen-induced arthritis in DBA/1 mice. Mice were primed (day −21) and challenged (day 0) with collagen and injected with anti-ST2L antibody or normal rabbit IgG daily from day 1. (a) Incidence rate (percent), (b) clinical score, and (c) number of arthritic paws. Data are mean ± SEM, n = 15. Mice (n = 4) were killed 48 h after antibody treatment, spleen cells were pooled and cultured with collagen (50 μg/ml) for 48– 96 h, and culture supernatants were assayed for cytokines by ELISA. *P <0.05, **P <0.01 (two sample t test). IL-4 and IL-5 were not detected (<20 pg/ml). T cell proliferation was expressed as [³H]thymidine uptake in triplicate cultures (mean cpm × 10³ ± SEM): antibody treated, 52.8 ± 6.8; control, 27.8 ± 1.1 (P <0.05).