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. 1998 Mar 2;187(5):733–741. doi: 10.1084/jem.187.5.733

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Detection of MCP-1 mRNA expression in mucosal lymphoid tissue after antigen feeding. Mice were fed either PLP139–151 or control peptide and both Peyer's patch and MLNs were harvested and assayed for MCP-1 mRNA expression using RT-PCR. Tissue was harvested at the indicated time points (in days) after oral antigen administration. Control MCP-1 mRNA expression (C) was determined from LPS-stimulated macrophages and used as an internal control for the RT-PCR conditions. Amplified cDNA was electrophoresed through 2% agarose gels and visualized using ethidium bromide. The data are representative of three identical experiments.