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. 1998 Mar 16;187(6):897–902. doi: 10.1084/jem.187.6.897

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Fas stimulation in NPD lymphoblasts efficiently induces PC-PLC activity, but fails to generate GD3 ganglioside accumulation. Cell extracts from 10⁷ normal (A) and NPD (B) lymphoblasts treated for 15 min with control IgM or 500 ng/ml anti-Fas mAb, preceded or not by preincubation with 50 μg/ml D609 for 30 min, were reacted with radiolabeled PC, and the released PCho was quantitated. (C) 10⁷ normal and NPD lymphoblasts were left untreated or were stimulated with 100 μM C₆-ceramide (cer) or 500 ng/ml anti-Fas for 5 or 15 min, respectively. Then polar lipids were extracted and run on HPTLC plates along with GD3 standard (st), and GD3 was detected by anti-GD3 immunostaining. Four experiments gave comparable results.