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. 1998 Mar 16;187(6):847–853. doi: 10.1084/jem.187.6.847

Figure 2.

Figure 2

Figure 2

Antigen receptor cross-linking induces Fas resistance in tolerant B cells. (A) Primary B cells from wild-type (WT, C57BL/6J), STg, or DTg mice were stimulated with either CD40L–CD8α fusion protein cross-linked with CD8-specific antibody (black bars), or CD40L plus F(ab′)2 fragments of goat anti–mouse IgM at 10 μg/ml (hatched bars), for 16 h as indicated. B cells were then radiolabeled and tested for susceptibility to Fas-dependent lysis in standard 4-h lectin-dependent 51Cr–release assays using AE7 CD4+ Th1 effector cells. Results for E/T cell ratios of 9:1 are presented. For each condition, the mean percentage of specific lysis of triplicate assays is displayed, along with a line indicating the SEM. One of three comparable experiments is shown. (B) Primary B cells from STg or DTg mice were stimulated with either CD40L (black bars) or CD40L plus anti-IgD at 100 ng/ml (hatched bars) for 16 h as indicated. B cells were then radiolabeled and tested for susceptibility to Fas-dependent lysis as above. Results for E/T cell ratios of 9:1 are presented as described above.