Figure 5.

Increased Stat3 activation in extracts of lungs of animals subjected to hemorrhagic shock and resuscitation. In A, EMSA was performed using the hSIE duplex oligonucleotide and 20 μg of extracts from shock animals (hemorrhagic shock, HS) or sham animals treated or untreated with L-NIL. The position of the SIF-A, -B, and -C complex are indicated. In B, the SIF-A band was quantitated by PhosphorImager analysis and the mean ± SEM for each group plotted. The mean of the SIF-A complexes in the shock group was 4.7-fold greater than in sham controls (P = 0.002) and was significantly reduced by L-NIL treatment (P = 0.04). In C, extracts of a representative lung were incubated with antibodies specific for Stat3α, Stat3β, or with both antibodies. The position of the SIF-A, -B, and -C complexes and the residual SIF-A complex after supershift of Stat3α and Stat3β (Stat3γ) are indicated.