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. 1998 Mar 16;187(6):855–864. doi: 10.1084/jem.187.6.855

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Kinetics of the C3H/HeJBir CD4⁺ T cell response to enteric bacterial antigens. 4 × 10⁵ spleen and MLN CD4⁺ T cells from C3H/ HeJBir mice of various ages from 2 to 25 wk were cultured with 4 × 10⁵ cecal bacterial (closed circles) or E. coli (open circles) antigen pulsed APCs from C3H/HeJ mice per well, prepared as in Fig. 1. 0.5 μCi of [³H]TdR was added to the wells for the last 18 h of a 5-d culture. Results are expressed as mean cpm ± SD of triplicate cultures.