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. 1998 Mar 16;187(6):885–895. doi: 10.1084/jem.187.6.885

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Kinetics of NP-binding IgG₁ AFCs and serum IgG₁ antibody elicited by primary immunization with NP–CG. Numbers of NP-binding AFCs from BM (open circles) and spleen (filled circles) were determined by ELISPOT using NP₂₆–BSA as the capture antigen (A). NP-binding serum antibody was determined by ELISA using NP₂₆–BSA (B). The data represent the mean ± SD from three to nine individual mice per time point.