Figure 5.

Degradation of TCR-α in BW5147. ³⁵S-labeling of BW5147 cells and chase was carried out as described in Figure 2. Samples were lysed in Triton X-100 lysis buffer with 0.1% SDS to decrease nonspecific binding. Anti–TCR-α (A and C) immunoprecipitates were washed in 0.1% Triton X-100, 0.1% SDS and resolved by SDS-PAGE under reducing conditions (A) or on two-dimensional nonreducing/reducing gels (C) followed by autoradiography. The longer exposure of the left upper panel of C is to demonstrate the position of disulfide-linked TCR-α/β dimers. B shows a quantitation of the experiment in A. Quantitation was by Storm 820 PhosphorImager using ImageQuant software.