Figure 9.
Ubiquitination of TCR-α in BW5147. (A) Cells were treated with or without LLnL as indicated and fractionated as described for Fig. 4. 106 cells were used in the whole cell lysate lanes (W ), and 5 × 107 cells were fractionated into cytosol and membrane fractions. Lysis and washing of immunoprecipitates was in buffer supplemented with 0.1% SDS. Immunoblotting was with anti-Ub. (B) LLnL-treated cells were broken and treated with or without proteinase K followed by immunoprecipitation and anti-Ub immunoblotting.