Figure 9.

Ubiquitination of TCR-α in BW5147. (A) Cells were treated with or without LLnL as indicated and fractionated as described for Fig. 4. 10⁶ cells were used in the whole cell lysate lanes (W  ), and 5 × 10⁷ cells were fractionated into cytosol and membrane fractions. Lysis and washing of immunoprecipitates was in buffer supplemented with 0.1% SDS. Immunoblotting was with anti-Ub. (B) LLnL-treated cells were broken and treated with or without proteinase K followed by immunoprecipitation and anti-Ub immunoblotting.