Figure 4.

Both fresh monocytes and non-Langerhans DCs differentiate toward either DCs or LCs depending on the absence or presence of TGF-β1, and retrieval of TGF-β1 did not result in loss of the Langerhans phenotype. Dot plots represent double immunostaining with CD1a and CD14 antibodies at days 0, 6, and 9 of culture; histogram plots represent E-cadherin expression at the same time (thick lines); and thin lines represent isotypic control staining. Only TGF-β1–supplemented cultures gave rise to E-cadherin+ DCs. Late addition of TGF-β1 in day 6 DC cultures also resulted in expression of E-cadherin. Removal of TGF-β1 at day 6 did not result in downregulation of E-cadherin expression. Data are representative of six experiments.