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. 1998 Apr 6;187(7):1081–1091. doi: 10.1084/jem.187.7.1081

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BrdU incorporation and PI analysis of anti-IgM–stimulated B cells from CBA/Ca and xid B mice. The diagram at the top indicates the cell cycle position of cells at the time the cells were harvested. This analysis identifies cells in G0 (G0), S phase (S), and those which have completed S phase and are now in the second cell cycle G0 phase (2nd G0), and apoptotic cells (Apo). B cells from CBA/Ca (top row) and xid (bottom row) mice were stimulated with 25 μg/ml of anti-IgM in the presence of BrdU. Cells were harvested at time points indicated and fixed to permit intranuclear staining of DNA with PI. The initial entry of B cells from xid and CBA/Ca is indicated with an arrow. Analysis was performed on FACSCalibur^® using CellQuest software.