Figure 2.

Y. enterocolitica inhibits prolonged degradation of IκB-α and IκB-β in J774A.1 macrophages. The kinetics of degradation of IκB-α and IκB-β was determined in untreated J774A.1 macrophages (lane 1) and in macrophages stimulated with LPS (lane 2), with the virulence plasmid–cured Yersinia strain WA-C (lane 3), and with the virulent wild-type strain WA-314 (lane 4). The cytoplasmic lysates of stimulated J774A.1 cells were prepared at the time points indicated, subjected to SDS-PAGE, and immunoblotted with polyclonal anti–IκB-α and anti–IκB-β antibodies, respectively.