Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Apr 6;187(7):985–996. doi: 10.1084/jem.187.7.985

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Generation of p105^−/− mice. (A) Targeting strategy of the ankyrin-encoding region of the nfkb1 gene. The relevant structure of the murine nfkb1 gene is shown at the top. Targeting vector pPNT/IκBγ II and the targeted allele are shown at the middle and bottom, respectively. Closed boxes, exons of nfkb1 gene; open boxes, the SV40 p(A), phosphoglycerate kinase (PGK)-neo, and PGK-tk cassettes. The position of EcoRI and EcoRV sites are indicated by I and V, respectively. The diagnostic restriction fragments used for Southern blot analysis are indicated at the top (wild-type allele) and bottom (targeted allele). The DNA fragments used as 5′ external (EE), 5′ internal (XX), 3′ internal (E), and neo-specific (PP620) probes are indicated at the bottom. (B) Genotype analysis of pups generated from p105^+/− intercrosses. Tail DNAs were digested with EcoRI, followed by Southern blot analysis using the 5′ internal probe XX. The wild-type allele is indicated by a 11.2-kb band, whereas the recombinant allele is represented by a 6.5-kb band. (C) Lack of p105 in homozygous mutants. Whole tissue extracts from thymus were subjected to Western blot analysis using a p50 antibody. Specific signals for the p50 isoform, p50, and p105 proteins are indicated by arrows.

Generation of p105^−/− mice. (A) Targeting strategy of the ankyrin-encoding region of the nfkb1 gene. The relevant structure of the murine nfkb1 gene is shown at the top. Targeting vector pPNT/IκBγ II and the targeted allele are shown at the middle and bottom, respectively. Closed boxes, exons of nfkb1 gene; open boxes, the SV40 p(A), phosphoglycerate kinase (PGK)-neo, and PGK-tk cassettes. The position of EcoRI and EcoRV sites are indicated by I and V, respectively. The diagnostic restriction fragments used for Southern blot analysis are indicated at the top (wild-type allele) and bottom (targeted allele). The DNA fragments used as 5′ external (EE), 5′ internal (XX), 3′ internal (E), and neo-specific (PP620) probes are indicated at the bottom. (B) Genotype analysis of pups generated from p105^+/− intercrosses. Tail DNAs were digested with EcoRI, followed by Southern blot analysis using the 5′ internal probe XX. The wild-type allele is indicated by a 11.2-kb band, whereas the recombinant allele is represented by a 6.5-kb band. (C) Lack of p105 in homozygous mutants. Whole tissue extracts from thymus were subjected to Western blot analysis using a p50 antibody. Specific signals for the p50 isoform, p50, and p105 proteins are indicated by arrows.