Figure 5.

i-IEL development in ζ/η^−/−–Fcγ^−/− mice i-IELs were prepared from mice as described (13) and three-color FCM was performed. For internal staining, cells were first stained with anti-CD4 and anti– CD8-β externally, then treated with intracellular staining buffer followed by staining with anti-CD3, anti–TCR-β, or anti–TCR-δ mAbs. Data depict two-color analysis of CD3 versus TCR-β or CD3 versus TCR-δ on software-gated CD4⁻ CD8-β⁻ cells. Numbers reflect the percentage of gated CD4⁻CD8-β⁻ cells in that quadrant.