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. 1998 Apr 6;187(7):1093–1101. doi: 10.1084/jem.187.7.1093

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Phenotype of DETC and DN NK1.1⁺ thymocytes from ζ/η^+/+–Fcγ^+/+, ζ/η^−/−–Fcγ^+/+, ζ/η^+/+–Fcγ^−/−, and ζ/η^−/−–Fcγ^−/− mice. (A) DETCs were purified as described in Materials and Methods and stained with antibodies directed against TCR-γδ and CD3-ε or Thy 1 and CD45. (B) Expression of surface TCR on NK1.1⁺ thymocytes from ζ/η^+/+–Fcγ^+/+, ζ/η^−/−–Fcγ^+/+ and ζ/η^−/−–Fcγ^−/− mice. Shown are two-color plots of NK1.1 versus. α/β-TCR or γ/δ-TCR on gated (CD24⁻) thymocytes. Numbers in quadrants refer to percent of NK1.1⁺ thymocytes that express TCR (α/β-TCR or γ/δ-TCR). Single-color plots show expression of α/β-TCR on gated NK1.1⁺ thymocytes. Shaded areas in single color histograms represent staining with negative control antibodies.

Phenotype of DETC and DN NK1.1⁺ thymocytes from ζ/η^+/+–Fcγ^+/+, ζ/η^−/−–Fcγ^+/+, ζ/η^+/+–Fcγ^−/−, and ζ/η^−/−–Fcγ^−/− mice. (A) DETCs were purified as described in Materials and Methods and stained with antibodies directed against TCR-γδ and CD3-ε or Thy 1 and CD45. (B) Expression of surface TCR on NK1.1⁺ thymocytes from ζ/η^+/+–Fcγ^+/+, ζ/η^−/−–Fcγ^+/+ and ζ/η^−/−–Fcγ^−/− mice. Shown are two-color plots of NK1.1 versus. α/β-TCR or γ/δ-TCR on gated (CD24⁻) thymocytes. Numbers in quadrants refer to percent of NK1.1⁺ thymocytes that express TCR (α/β-TCR or γ/δ-TCR). Single-color plots show expression of α/β-TCR on gated NK1.1⁺ thymocytes. Shaded areas in single color histograms represent staining with negative control antibodies.