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. 1998 Apr 6;187(7):1057–1067. doi: 10.1084/jem.187.7.1057

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Anti-CD3ε stimulation of perforin/granule exocytosis and FasL/Fas killing. 14-7 and 14-7FD were incubated with either mock treated or peptide antigen HA529–537 (0.01 μM) pulsed target cells at an effector to target ratio of 5:1. For anti-CD3ε wells, soluble 145-2C11 (5 μg/ml) was added or the plate was coated with 5 μg/ ml 145-2C11 for 30 min at room temperature before addition of the CTL or the target. (a) ⁵¹Cr cytolysis assay with L1210Fas- target. (b) BLT-esterase assay with supernatants from a and from CTL stimulated in the absence of target cells. (c) ⁵¹Cr release assay with L1210Fas⁺ target.

Anti-CD3ε stimulation of perforin/granule exocytosis and FasL/Fas killing. 14-7 and 14-7FD were incubated with either mock treated or peptide antigen HA529–537 (0.01 μM) pulsed target cells at an effector to target ratio of 5:1. For anti-CD3ε wells, soluble 145-2C11 (5 μg/ml) was added or the plate was coated with 5 μg/ ml 145-2C11 for 30 min at room temperature before addition of the CTL or the target. (a) ⁵¹Cr cytolysis assay with L1210Fas- target. (b) BLT-esterase assay with supernatants from a and from CTL stimulated in the absence of target cells. (c) ⁵¹Cr release assay with L1210Fas⁺ target.