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. 1998 Apr 6;187(7):1057–1067. doi: 10.1084/jem.187.7.1057

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Anti-CD3ε induction of Ca²⁺ mobilization and influx in 14-7 and 14-7FD. Anti-CD3ε (145-2C11) (45 μg/ml) was used to trigger 14-7 and 14-7FD Ca²⁺ mobilization, and influx was monitored by indo-1 fluorescence. EGTA (5 mM) was used to examine the intra- and extracellular release components of the Ca²⁺ signaling pathways in 14-7 (c, e) and 14-7FD (d, f ). EGTA was added before (c and e) or after (d and f ) anti-CD3ε addition.