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. 1998 Apr 20;187(8):1249–1260. doi: 10.1084/jem.187.8.1249

Figure 1.

Figure 1

CD3fos-F(ab′)2 (CD3/CD3) induces the differentiation of mature CD8+ cells and antagonizes the normal differentiation of CD4+ T cells in neonatal thymus lobes. Newborn thymus lobes from (A) MHC class I–restricted F5/RAG-1−/β2m− transgenic (24); (B) class II–restricted A18/RAG-1− transgenic (25); (C) β2m−; and (D) MHC− (class II−β2m−) mice were cultured with 5 μg/ml (A and B), 50 μg/ml (C), or 20 μg/ml (D) CD3fos-F(ab′)2 (CD3/CD3) or CD8jun-F(ab′)2 (CD8/CD8). Dot-plots show CD8 and CD4 expression of all thymocytes recovered from a single lobe. The percentage of total cells for individual subpopulations are indicated. HSA levels of CD4+ (thick line), DP (light fill) and CD8+ (dark fill) subpopulations are presented in histograms below each dot-plot. The data are representative of 13 (A), 8 (B), 6 (C), and 4(D) independent experiments.