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. 1998 Apr 20;187(8):1355–1360. doi: 10.1084/jem.187.8.1355

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Requirement of cytoplasmic tyrosine residues in inhibitory response. (A) Calcium mobilization was monitored after BCR cross-linking (solid line) and coligation (dashed line) in IIA1.6 cells expressing various FcγRIIB–PIR-B mutants. Surface expression levels of FcγRIIB–PIR-B mutants are indicated in inset boxes. (B) Comparison of inhibitory effect by various FcγRIIB–PIR-B mutants. The percentage of [Ca²⁺]_i was given by the total calcium mobilization elicited by coligation of BCR to FcγRIIB–PIR-B over that by BCR stimulation alone. The results are expressed as the mean from two independent clones.