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. 1998 Apr 20;187(8):1355–1360. doi: 10.1084/jem.187.8.1355

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Reduction of FcγRIIB–PIR-B–mediated inhibitory effect in SHP-1/SHP-2 double-deficient cells. (A) SHIP, SHP-1, or SHP-2 protein expression on various gene-targeted DT40 cells. Each protein was immunoprecipitated and was detected by Western blotting analysis using anti-SHIP, anti–SHP-1, or anti–SHP-2 Ab. (B) Calcium mobilization was measured after BCR cross-linking (solid line) and coligation (dashed line) in various DT40 mutants expressing FcγRIIB–PIR-B. Surface expression levels of FcγRIIB–PIR-B are indicated in inset boxes. (C) Comparison of inhibitory effect in various mutant DT40 cells. The results are expressed as the mean from three independent clones. Error bars represent SD from the mean.