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. 1998 Apr 20;187(8):1235–1247. doi: 10.1084/jem.187.8.1235

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FcεRI-induced histamine release and cytokine secretion from wt and xid-BMMCs. BMMCs were sensitized by overnight incubation with 1 μg/ml anti-DNP monoclonal IgE antibody. Cells resuspended in Tyrode solution (A and B) or culture media (C) were stimulated by the indicated concentrations of DNP-HSA for 45 min (A and B) or 24 h (C). Histamine released into the buffer was measured by an automatic fluorometric assay (A and B). Cytokines secreted into the culture media during 24 h were measured by ELISA (C). The results of histamine release are mean values of duplicates per point, with SEM <5% of the mean. The results shown in A for xid-BMMCs and their corresponding wt-BMMCs are representative of those obtained in more than five experiments. The results shown in B are representative of those from two experiments. Cytokine measurements shown in C were performed in triplicate and mean values are shown, with SEM <10% of the mean. The results in C are representative of those from at least seven experiments.

FcεRI-induced histamine release and cytokine secretion from wt and xid-BMMCs. BMMCs were sensitized by overnight incubation with 1 μg/ml anti-DNP monoclonal IgE antibody. Cells resuspended in Tyrode solution (A and B) or culture media (C) were stimulated by the indicated concentrations of DNP-HSA for 45 min (A and B) or 24 h (C). Histamine released into the buffer was measured by an automatic fluorometric assay (A and B). Cytokines secreted into the culture media during 24 h were measured by ELISA (C). The results of histamine release are mean values of duplicates per point, with SEM <5% of the mean. The results shown in A for xid-BMMCs and their corresponding wt-BMMCs are representative of those obtained in more than five experiments. The results shown in B are representative of those from two experiments. Cytokine measurements shown in C were performed in triplicate and mean values are shown, with SEM <10% of the mean. The results in C are representative of those from at least seven experiments.

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