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. 1998 Apr 20;187(8):1235–1247. doi: 10.1084/jem.187.8.1235

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Transcriptional activity of luciferase reporter constructs in wt mast cells or btk null mast cells reconstituted with wt or K430R btk cDNAs. Promoter reporter constructs, IL-2 (−321)–luc, TNF-α (−200)–luc, NFAT–luc, NFκB–luc, or c-fos–luc, were transfected into wt or btk null mast cells (A) or btk null mast cells reconstituted with vector, wt btk, or K430R btk retroviruses (B). Luciferase activity was measured in cell lysates that were prepared 8 h after antigen stimulation or mock stimulation. Fold activities in FcεRI-stimulated cells versus unstimulated cells (= 1) are shown. The results shown are representative of those obtained in at least three experiments of each type.

Transcriptional activity of luciferase reporter constructs in wt mast cells or btk null mast cells reconstituted with wt or K430R btk cDNAs. Promoter reporter constructs, IL-2 (−321)–luc, TNF-α (−200)–luc, NFAT–luc, NFκB–luc, or c-fos–luc, were transfected into wt or btk null mast cells (A) or btk null mast cells reconstituted with vector, wt btk, or K430R btk retroviruses (B). Luciferase activity was measured in cell lysates that were prepared 8 h after antigen stimulation or mock stimulation. Fold activities in FcεRI-stimulated cells versus unstimulated cells (= 1) are shown. The results shown are representative of those obtained in at least three experiments of each type.