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. 1998 Apr 20;187(8):1335–1342. doi: 10.1084/jem.187.8.1335

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Serum levels of anti-Gal NAb in lethally irradiated GalT KO mice. Peripheral blood samples (40 μl) were collected from lethally irradiated (9.75 Gy) GalT KO mice that did (⋄) or did not (•) receive TCD GalT KO BMC (5 × 10⁶ cells/mouse), and from 9.75 Gy–irradiated WT mice (□) at the indicated times, and serum levels of anti-Gal NAb were measured by FCM. WT scid mouse cells were stained with 10 μl serum, and NAbs were detected using rat anti–mouse IgM-FITC as secondary mAb. The anti-Gal NAb (NAB) levels are presented as median fluorescence intensity (MFI). Each line represents an individual animal.