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Effect of eIF1 and eIF1A on 48S complex formation. MC SPV9 (CAA) (A) and MC SPV9 SL− (CAA) (B,C) mRNAs were incubated with 40S subunits, eIF2, eIF3, eIF1, eIF1A, eIF4A, eIF4B, eIF4F, and Met-tRNAi Met as indicated, in standard conditions. Extension by AMV-RT of a primer annealed to the NS′ cistron was arrested at sites indicated on the right as positions relative to the A (position +1) of the initiation codon. Reference lanes A, T, G, and C depict the SPV9 (CAA) sequence.
