FIGURE 6.

Recombinant KREPA6 has a higher affinity for poly(U) RNA. (A) EMSA analysis of HisKREPA6 binding to synthetic 24-mer RNAs. (B) Quantitation of the RNA binding in A indicating the percent of RNA bound with increasing HisKREPA6 concentrations. The RNA ligands were 5′-end-labeled (UC)₁₂ (white bars), (AG)₁₂ (striped bars), (UG)₁₂ (gray bars), and (UU)₁₂ (black bars). Owing to the apparent RNA degradation in lanes containing high concentrations of HisKREPA6, the amount of radioactive free RNA in the absence of HisKREPA6 (lane 11 of each gel) was used to estimate percent binding as detailed in the Materials and Methods. Also because of this RNA degradation, apparent K _D estimations could not be accurately determined.