Table 1.
Tetrameric Mamu-A*01/p11C, C–M Complex Binding to T Cells Correlates with p11C-specific CTL Activity*
| Monkey | Percent CD8α/β+ T cells stained with Mamu-A*01/p11C, C–M‡ | p11C-specific lysis§ (Percentage) | pCTL¶ (per 106 cells) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Whole blood | Con A/IL-2 expanded | p11C-pulsed APC expanded | Con A/IL-2 expanded (80.1)‖ | p11C-pulsed APC expanded (10.1)‖ | ||||||||
| 403 | 10.3 | 22.4 | 86.3 | 73 | 74 | 279 | ||||||
| 138 | 2.1 | 2.1 | 25.8 | 31 | 69 | 46 | ||||||
| 297 | 0.9 | 1.5 | 37.2 | 28 | 61 | <3 | ||||||
| 348 | 0.7 | 0.5 | 5.1 | 22 | 15 | <3 | ||||||
*
p11C, C–M represents the optimal 9–amino acid fragment of the SIV Gag 12–amino acid peptide p11C (12).
‡
Staining with FITC-coupled tetrameric Mamu-A*01/p11C, C–M complex on gated CD8α/β T cells.
§
Percent p11C-specific lysis was calculated as percent specific release by p11C-pulsed MHC class I–matched target cells minus the average of the percent specific release by control peptide p11B-pulsed target cells.
‖
E/T ratio.
¶
CTL precursor frequency for p11C per 106 PBMCs assessed by LDA.