Figure 7.

B10.A(3R) mice are tolerant to Eα52–68, but not to Eα52–66, whereas C57BL/6 mice can be primed with both peptides. The proliferative response of whole lymph node cultures from 3R mice immunized with 30 μg Eα52–68 (A) or Eα52–66 (B). Popliteal lymph node cells from two mice per experiment were pooled. 4 × 10⁵ cells were cultured with various doses of peptide for 60–72 h before pulsing overnight with 1 μCi/well of [³H]thymidine. The data are representative of several experiments. However, the response of mice immunized with Eα52–66 was low, comparable to that of mice immunized with Eα52–68, in two of five experiments. The response of mice immunized with Eα52–68 was very weak in all experiments. The response of B6 mice immunized with Eα52–68 (C) and Eα52–66 (D) is shown. B6 mice responded similarly in response to both peptides in all experiments, although typically, as here, the response to Eα52–66 was greater than the response to Eα52–68.