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. 1998 May 4;187(9):1451–1461. doi: 10.1084/jem.187.9.1451

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Biosynthetic characteristics of KL-1 cytoplasmic domain variants and KL-Sl ^17H. COS-1 cells were transfected with various mutant constructs, KL-1, KL-L263, KL-N254, KL-Δ241/254, KL-Sl ^17H, and KL-Q241, labeled with ³⁵S-methionine for 30 min, and chased in regular medium. Cell lysates were collected at designated time points, immunoprecipitated with anti-KL antibody, and analyzed by SDS-PAGE (12%). Arrowheads, Mature glycosylated KL proteins. *Immature forms. Molecular mass (M_r) markers are indicated in kilodaltons.