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. 1998 May 4;187(9):1451–1461. doi: 10.1084/jem.187.9.1451

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KL protein expression detected by immunofluorescence in COS-1 cells expressing wild-type and mutant KL protein products. COS-1 cells transfected with KL-1, KL-Q241, and KL-Sl ^17H grown on coverslips were fixed with 2% formaldehyde (NP, nonpermeabilized control) and permeabilized with 0.1% saponin (P). The cells were incubated with anti-KL antibody or an ER marker antibody and FITC-conjugated goat anti– rabbit antibody. Photographs were taken with fluorescent microscopy.