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. 1998 May 4;187(9):1451–1461. doi: 10.1084/jem.187.9.1451

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Demonstration of KL-1, KL-2 dimers in transfected COS-1 cells. COS-1 cells were transfected with various normal and mutant KL-1 and KL-2 constructs: (A) KL-1, KL-2, and KL-S; (B) KL-1: L263, V248, Δ241/254; KL-2: L263, N254, Q241. 72 h after transfection, they were treated with the cross-linkers DSS and BS³ for 1 h at 37°C. Cell lysates were collected and fractionated by SDS-PAGE (12%), and KL protein products were identified by Western blotting. Arrowheads, KL dimers. *Monomers. Molecular mass (M_r) markers are indicated in kilodaltons.