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. 1998 May 4;187(9):1451–1461. doi: 10.1084/jem.187.9.1451

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Adhesion of BMMCs to COS-1 cells expressing KL-1, secretory KL-1S, different KL cytoplasmic variants, and KL-Sl ^17H. COS-1 cells were transferred to 24-well plates 24 h after transfection at a density of 7.5 × 10⁴ cells per well. BMMCs (1.5 × 10⁵ cells per well) were incubated with transfected COS-1 cells for 1 h in the presence and absence of anti–c-kit antibody. Nonattached BMMCs were washed away three times with medium, and the number of attached BMMCs was counted by microscopic inspection. The number of BMMCs attached to individual COS cells is shown.