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. 1998 May 18;187(10):1659–1669. doi: 10.1084/jem.187.10.1659

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Binding of H. pylori to gastric epithelium correlates with class II MHC expression. Sections obtained from gastric antral biopsy from H. pylori–infected subjects were examined for the expression of class II MHC molecules by immunohistochemistry or the appropriate isotype control as described in Materials and Methods. Although no staining was detected with the isotype control, the photomicrograph in A demonstrates that specific antibodies detect class II MHC on gastric epithelial cells and that staining can be detected on the apical surface (arrows in lumen). Examples of the lumen are indicated (L); ×400. To document the correlation between class II MHC expression and the binding of H. pylori, gastric epithelial cells were isolated from biopsy specimens collected from an infected subject. Subsequently, class II MHC expression and H. pylori binding were measured simultaneously by flow cytometry. The data in B show the correlation of the fluorescence intensity for the two parameters (r ² = 0.93, P <0.05). Representative of four separate experiments.

Binding of H. pylori to gastric epithelium correlates with class II MHC expression. Sections obtained from gastric antral biopsy from H. pylori–infected subjects were examined for the expression of class II MHC molecules by immunohistochemistry or the appropriate isotype control as described in Materials and Methods. Although no staining was detected with the isotype control, the photomicrograph in A demonstrates that specific antibodies detect class II MHC on gastric epithelial cells and that staining can be detected on the apical surface (arrows in lumen). Examples of the lumen are indicated (L); ×400. To document the correlation between class II MHC expression and the binding of H. pylori, gastric epithelial cells were isolated from biopsy specimens collected from an infected subject. Subsequently, class II MHC expression and H. pylori binding were measured simultaneously by flow cytometry. The data in B show the correlation of the fluorescence intensity for the two parameters (r ² = 0.93, P <0.05). Representative of four separate experiments.