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. 1998 Jun 1;187(11):1753–1765. doi: 10.1084/jem.187.11.1753

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Identification of ERK2 as the activated MAPK isoform in Raji-triggered YT effector cells. (A) YT cells untreated or pretreated 1 h at 37°C with 100 μM of PD098059 or an equivalent amount of the diluent, DMSO, were mixed with Raji tumor cells at a 1:1 ratio for 0–5 min at 37°C. The cells were then lysed and immunoprecipitated (IP) with antiphosphotyrosine, 4G10, followed by Western blot analysis (WB) with anti-ERK2. MED, Medium. (B) YT cells similarly treated as in A were immunoprecipitated with anti-AMAPK (α-AMAPK) and probed with the same antibody to locate the activated phosphorylated form of MAPK. The filter was then stripped and reprobed with anti-ERK2 to identify the activated MAPK isoform as ERK2. NRS, Normal rabbit serum.

Identification of ERK2 as the activated MAPK isoform in Raji-triggered YT effector cells. (A) YT cells untreated or pretreated 1 h at 37°C with 100 μM of PD098059 or an equivalent amount of the diluent, DMSO, were mixed with Raji tumor cells at a 1:1 ratio for 0–5 min at 37°C. The cells were then lysed and immunoprecipitated (IP) with antiphosphotyrosine, 4G10, followed by Western blot analysis (WB) with anti-ERK2. MED, Medium. (B) YT cells similarly treated as in A were immunoprecipitated with anti-AMAPK (α-AMAPK) and probed with the same antibody to locate the activated phosphorylated form of MAPK. The filter was then stripped and reprobed with anti-ERK2 to identify the activated MAPK isoform as ERK2. NRS, Normal rabbit serum.