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. 1998 Jun 1;187(11):1813–1823. doi: 10.1084/jem.187.11.1813

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CD4, CD8, and TCR-α/β profiles of bone marrow cells before and after culture. Freshly isolated bone marrow cells from C57BL/Ka (A) or BALB/c (F) mice were depleted of cells expressing CD4, CD8, and TCR-α/β markers using the gating boxes in the lower left and reanalyzed in B and G. The depleted marrow cells were cultured for 48 h and the bright TCR-α/β⁺ cells that developed are enclosed in the boxes in C and H. The depleted cultured cells were analyzed for CD4 versus CD8 markers in D and I. Analyses of non–T cell–depleted marrow cells after culture are shown in E and J.