Figure 5.

Add back of marrow T cells to T cell–depleted marrow cultures. Freshly isolated marrow cells (A) were sorted using the gating boxes 1 and 2, and reanalysis of T-depleted cells in box 1 are shown in B. After 48 h of culture of cells in B, newly formed T cells are enclosed in the box in C. Culture of combined cells from boxes 1 and 2 are shown in D. Marrow cells were also sorted for CD4⁺ or CD8⁺ TCR-α/β⁺ (E, box 2), or CD4⁻CD8⁻ TCR-α/β⁺ (E, box 3) T cells. F and G show T cell-depleted marrow cells before and after culture. H and I show T cell-depleted marrow cells cultured in combination with cells from E, box 2 or 3, respectively. Marrow cells were also gated for NK1.1⁻ TCR-α/β⁻ (  J, box 1), NK1.1⁺ TCR-α/β⁺ (  J, box 2), and NK1.1⁻TCR-α/β⁺ (  J, box 3) subsets, respectively. Sorted NK1.1⁻TCR-α/β⁺ (K) cells were cultured alone (L), or with cells from box 2 (M) or box 3 (N).