Figure 6.

Detection of MHV-68 in organs of μMT mice after adoptive transfer of B lymphocytes. MHV-68 genomes were detected by nested PCR for gp150. Products were analyzed on 2% agarose gels containing ethidium bromide and visualized using a UV transilluminator. Images are shown with the colors reversed for clarity. Molecular weight determinations were made relative to a 1-kb DNA ladder (Mr), and the size of the pertinent bands in bp are shown at the left. In all panels, DNA from the MHV-68–positive tumor cell line S11 was used as a positive control (+VE), and produced the expected product of 368 bp. DNA from the MHV-68–negative tumor cell line S31 (−VE) was used along with deionized water (H₂O) as a negative control. (A) T-depleted splenocytes from infected (INF 1–4) or mock-infected (MOCK 1–3) C57/BL6 mice were adoptively transferred into uninfected μMT mice. After 11 d, DNA was extracted and analyzed for MHV-68 by nested PCR. Results from analysis of the spleens, lungs, and blood are indicated above the relevant tracks. (B) Three persistently infected μMT mice (1–3) were analyzed for MHV-68 by nested PCR. Results from either lungs or spleen are shown above the relevant tracks. (C) T cell–depleted splenocytes from uninfected C57/BL6 mice were adoptively transferred into either mock-infected (MOCK 1 and 2) or persistently infected (INF 1–3) μMT mice. After 14 d, DNA was extracted and analyzed for MHV-68 by nested PCR. Results from analysis of the spleens and lungs are indicated above the relevant tracks.