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. 1998 Jun 15;187(12):2031–2036. doi: 10.1084/jem.187.12.2031

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NFAT1 associates with HATs in nuclear extracts. Cl.7W2 T cells were stimulated with ionomycin and PMA for 25 min at 37°C, and nuclear extracts were immunoprecipitated (i.p.) with antibodies against the DBD (αDBD) or a COOH-terminal peptide (αCt) of NFAT1. An irrelevant antibody was used as a control. The immune complexes were tested for HAT activity as described in Materials and Methods. The results are representative of three independent experiments. Bars, The mean and range of duplicates in one such experiment.