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. 1998 Jun 15;187(12):1995–2007. doi: 10.1084/jem.187.12.1995

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RAS, RAF, and MAPK activation in BCR/ABL-expressing 32Dcl3 cells. (A) GTP-bound RAS was measured as described (39) using serum– and IL-3–starved (5 h) BCR/ABL- expressing cells. (B) c-Raf-1 activity was measured as described (40) using histone H1 as substrate. (C) c-Raf-1 tyrosine phosphorylation in BCR/ABL-expressing cells. Tyrosine phosphorylated c-Raf-1 was detected by antiphosphotyrosine blotting of anti–c-Raf-1 immunoprecipitates from BCR/ABL-expressing cells. (D) MAPK activity was measured as described (51), using MBP as substrate. Representative of three independent experiments.

RAS, RAF, and MAPK activation in BCR/ABL-expressing 32Dcl3 cells. (A) GTP-bound RAS was measured as described (39) using serum– and IL-3–starved (5 h) BCR/ABL- expressing cells. (B) c-Raf-1 activity was measured as described (40) using histone H1 as substrate. (C) c-Raf-1 tyrosine phosphorylation in BCR/ABL-expressing cells. Tyrosine phosphorylated c-Raf-1 was detected by antiphosphotyrosine blotting of anti–c-Raf-1 immunoprecipitates from BCR/ABL-expressing cells. (D) MAPK activity was measured as described (51), using MBP as substrate. Representative of three independent experiments.