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. 1998 Jun 15;187(12):1995–2007. doi: 10.1084/jem.187.12.1995

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c-Raf-1 and Bcl-2 expression in subcellular fractions of parental and BCR/ABL-expressing 32Dcl3 cells. (A) Western blots show c-Raf-1 levels in fractions enriched for mitochondria (HM), cytoplasmic membrane (LM), and cytoplasm (C) from parental and BCR/ABL-expressing cells. Cells were starved of IL-3–containing medium for 16 h. (B) Western blots show Bcl-2 levels in the mitochondrial fraction of parental and BCR/ABL-expressing cells cultured as described above. Levels of the subunit IV of mitochondrial COX were measured as control of equal loading.

c-Raf-1 and Bcl-2 expression in subcellular fractions of parental and BCR/ABL-expressing 32Dcl3 cells. (A) Western blots show c-Raf-1 levels in fractions enriched for mitochondria (HM), cytoplasmic membrane (LM), and cytoplasm (C) from parental and BCR/ABL-expressing cells. Cells were starved of IL-3–containing medium for 16 h. (B) Western blots show Bcl-2 levels in the mitochondrial fraction of parental and BCR/ABL-expressing cells cultured as described above. Levels of the subunit IV of mitochondrial COX were measured as control of equal loading.