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. 1998 Jun 15;187(12):1995–2007. doi: 10.1084/jem.187.12.1995

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(A) RAF expression in total extracts of p185ΔBCR/ M-Raf transfectants. Western blot shows expression of endogenous and mitochondria-targeted Raf-1 in total extracts of selected clones. (B) RAF expression in subcellular extracts of p185ΔBCR/ M-Raf transfectants. Western blot shows expression of endogenous (Raf-1) and mitochondria-targeted (M-Raf) Raf-1 in subcellular extracts from p185ΔBCR/M-Raf selected clones. Levels of the subunit IV of mitochondrial COX and of HSP90 were measured as control of equal loading and of appropriate subcellular fractionation. (C) Bcl-2 and BAD expression in total cell lysate of p185ΔBCR– and p185ΔBCR/ M-Raf–expressing cells. (D) BAD expression in subcellular fractions of p185WT–, p185ΔBCR–, and p185ΔBCR/M-Raf – expressing cells. Western blots show BAD expression in mitochondria- (HM), cytoplasmic membrane– (LM), and cytoplasm- (C) enriched fractions. Arrows, hypo- and hyperphosphorylated BAD. Cells were starved of IL-3–containing medium for 16 h. Levels of the subunit IV of mitochondrial COX and of HSP90 were measured as control of equal loading and of appropriate subcellular fractionation.