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. 1998 Jun 15;187(12):1995–2007. doi: 10.1084/jem.187.12.1995

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(A) BCR/ABL and RAF expression in 32Dcl3 cells expressing a kinase-deficient or triple mutant p185BCR/ABL. Western blots show expression of mutant BCR/ABL (KD, K671R kinase deficient; TM, Y177F/R552L/Y793F triple mutant) and RAF (endogenous and mitochondria targeted) in a representative clone per each transfectant. Levels of HSP90 were determined as control for protein loading. (B and C) Viability of mutant (KD and TM) p185BCR/ABL- and mutant (KD and TM) p185BCR/ABL/M-Raf transfectants in IL-3–deprived cultures. Numbers of viable cells from triplicate wells were estimated for each transfectant at the indicated times by trypan blue exclusion assay. Representative of three independent experiments using four different clones per each transfectant.

(A) BCR/ABL and RAF expression in 32Dcl3 cells expressing a kinase-deficient or triple mutant p185BCR/ABL. Western blots show expression of mutant BCR/ABL (KD, K671R kinase deficient; TM, Y177F/R552L/Y793F triple mutant) and RAF (endogenous and mitochondria targeted) in a representative clone per each transfectant. Levels of HSP90 were determined as control for protein loading. (B and C) Viability of mutant (KD and TM) p185BCR/ABL- and mutant (KD and TM) p185BCR/ABL/M-Raf transfectants in IL-3–deprived cultures. Numbers of viable cells from triplicate wells were estimated for each transfectant at the indicated times by trypan blue exclusion assay. Representative of three independent experiments using four different clones per each transfectant.