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. 1998 Dec 7;188(11):2019–2031. doi: 10.1084/jem.188.11.2019

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Changes in expression of mRNA encoding components of the MHC class II biosynthesis/processing pathway in fresh or GM-CSF– exposed RTDC. (A) cDNA was prepared from RTDCs either as fresh cells or after overnight culture in GM-CSF and used either undiluted (undil.) or diluted 1:5 or 1:25 in a PCR reaction using primers specific for the indicated MHC class II biosynthesis and antigen processing-associated proteins as described in Materials and Methods. (B) MHC class II component:β-actin mRNA band density ratios (1:5 cDNA dilution) for fresh (solid bars) and GM-CSF–exposed (white bars) RTDCs. One representative out of two experiments is shown. RT1.B, rat MHC class II; Calnx, calnexin; Ii, invariant chain; DMa/DMb, RT1.DMa/b.

Changes in expression of mRNA encoding components of the MHC class II biosynthesis/processing pathway in fresh or GM-CSF– exposed RTDC. (A) cDNA was prepared from RTDCs either as fresh cells or after overnight culture in GM-CSF and used either undiluted (undil.) or diluted 1:5 or 1:25 in a PCR reaction using primers specific for the indicated MHC class II biosynthesis and antigen processing-associated proteins as described in Materials and Methods. (B) MHC class II component:β-actin mRNA band density ratios (1:5 cDNA dilution) for fresh (solid bars) and GM-CSF–exposed (white bars) RTDCs. One representative out of two experiments is shown. RT1.B, rat MHC class II; Calnx, calnexin; Ii, invariant chain; DMa/DMb, RT1.DMa/b.