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. 1998 Dec 7;188(11):2019–2031. doi: 10.1084/jem.188.11.2019

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In vivo priming for OVA-specific IgG production by GM-CSF–exposed RTDCs. Sorted RTDCs or splenic DCs (SpDC) were incubated overnight at 37°C with 1 mg/ml OVA and 10 ng/ml GM-CSF, then transferred intravenously to syngeneic recipients, and serum IgG1, 2a, and 2b levels were determined as described in Fig. 6 A. Identical results for RTDCs were obtained using 90-min OVA-pulsing before overnight culture in GM-CSF (data not shown). Results are expressed as mean ± SEM for 9 (RTDC) or 10 (SpDC) animals. *P < 0.0001 compared with fresh RTDCs.