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. 1998 Dec 7;188(11):2019–2031. doi: 10.1084/jem.188.11.2019

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Expression of IL-10 and IL-12 by fresh and GM-CSF– exposed RTDCs. (A) cDNA prepared from freshly isolated or GM-CSF– exposed RTDCs was used either undiluted (undil.) or diluted 1:5 and 1:25 in PCR reactions for rat IL-10 and IL-12p35 and p40 mRNA as described in Materials and Methods. (B) IL-10/IL-12:β-actin mRNA band density ratios (1:5 cDNA dilution) for fresh RTDCs and RTDCs exposed to GM-CSF alone for 24 or 48 h or GM-CSF plus TNF or CD40L for 48 h only. Mean values ± SEM for three experiments are shown for fresh and GM-CSF–exposed RTDCs, and representative results of a single experiment are shown for GM-CSF plus TNF or CD40L.

Expression of IL-10 and IL-12 by fresh and GM-CSF– exposed RTDCs. (A) cDNA prepared from freshly isolated or GM-CSF– exposed RTDCs was used either undiluted (undil.) or diluted 1:5 and 1:25 in PCR reactions for rat IL-10 and IL-12p35 and p40 mRNA as described in Materials and Methods. (B) IL-10/IL-12:β-actin mRNA band density ratios (1:5 cDNA dilution) for fresh RTDCs and RTDCs exposed to GM-CSF alone for 24 or 48 h or GM-CSF plus TNF or CD40L for 48 h only. Mean values ± SEM for three experiments are shown for fresh and GM-CSF–exposed RTDCs, and representative results of a single experiment are shown for GM-CSF plus TNF or CD40L.