Table 2.
Surface Phenotype of Fresh and GM-CSF–exposed RTDCs*
| Marker | mAb | Fresh | Plus GM-CSF | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Percentage frequencies | MFI | Percentage frequencies | MFI | |||||||
| CD4 | W3/25 | 29.7 | 4.5 | 2.9 | 2.7 | |||||
| CD8 | OX8 | 2.0 | 3.6 | 2.5 | 6.0 | |||||
| CD11a | WT.1 | 13.9 | 3.7 | 19.4 | 7.2 | |||||
| CD11b/c | OX42 | 3.3 | 3.1 | 10.8 | 6.8 | |||||
| CD45 | OX1 | 5.9 | 3.3 | 8.0 | 3.0 | |||||
| CD54 | 1A-29 | 8.9 | 6.3 | 4.1 | 9.9 | |||||
| CD80/86 | CTLA-4 Ig | 0.8 | 2.2 | 54.8 | 38.6 | |||||
| MHC class I | OX27 | 18.7 | 5.4 | 62.0 | 10.2 | |||||
| MHC class II | OX6 | 100 | 11.5 | 100 | 55.1 | |||||
*
Lung digests were depleted of mφ and B cells as described in Materials and Methods and labeled with the indicated mAbs and GAM-PE (or CTLA-4 Ig and GAH-PE) followed by OX6-FITC. Percentage frequencies and mean fluorescence intensities (MFI) are shown for 5 × 103 OX6+ gated RTDCs using positive analysis regions set on isotype-matched controls.