Table 3.
Cell Proliferation, IFN-γ, and IL-4 Production in Ex Vivo Spleen Cell Cultures of DC Adoptive Transfer Recipients*
| Type of DC transferred | No. of animals | Proliferation | IFN-γ | IL-4 | ||||
|---|---|---|---|---|---|---|---|---|
| CPM × 10−3 | U/ml | U/ml | ||||||
| Fresh RTDCs | 5 | 3.0 ± 0.8 | 0.1 | 1.6 ± 0.39 | ||||
| RTDCs + GM-CSF | 7 | 36.3 ± 2.2 | 10.2 ± 2.5 | 0 | ||||
| Spleen DCs | 9 | 69.4 ± 4.1 | 14.8 ± 3.3 | nt | ||||
| Control** | 8 | 3.8 ± 0.6 | 0.1 | nt |
*
Each animal intravenously received 105 OVA-pulsed DCs in 1 ml PBS on day 0 and 10 μg OVA on day 5. After 15 d, single cell suspensions of whole spleen were stimulated in vitro with 50 μg/ml OVA and analyzed for cell proliferation by [3H]thymidine incorporation, IFN-γ by ELISA, and IL-4 by bioassay after 72 h of culture. Results are expressed as mean ± SEM. nt, not tested.
**
Controls intravenously received 1 ml PBS on day 0 and 10 μg OVA on day 5.